Application of Beta-Glucuronidase Transient Expression for Selection of Maize Genotypes Competent for Genetic Transformation

Nitovska, I.O. and Abraimova, O.Ye. and Duplij, V.P. and Derkach, K.V. and Satarova, T.M. and Rudas, V.A. and Cherchel, V.Yu. and Dziubetskyi, B.V. and Morgun, B.V. (2019) Application of Beta-Glucuronidase Transient Expression for Selection of Maize Genotypes Competent for Genetic Transformation. Cytology and Genetics, 6. pp. 451-458. ISSN 1817-7883

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Abstract

Genetic transformation of inbred maize lines and F1 hybrids registered in Ukraine has been carriedout. The study employed a biolistic method for genetic transformation of immature maize embryos thatformed callus tissue and the pAHC25 vector containing the genes of phosphinothricin-N-acetyltransferase(bar) and β-glucuronidase (uidA). As a result of the transformation of callus tissue of maize genotypes, linesresistant to phosphinothricin and regenerated plants were obtained. The activity of β-glucuronidase in herbicide-resistant calli was detected. The presence of the bar gene in callus DNA was demonstrated by the PCRmethod. The rate of stable transformation ranged from 2.2 to 30% depending on the genotype. The relationshipbetween the results of transient expression of the β-glucuronidase gene and stable genetic transformationwas observed. The proposed protocol for genetic transformation of maize using the study of transient expressionof the β-glucuronidase gene makes it possible to significantly simplify the process of selecting genotypescompetent for genetic transformation and create transgenic organisms with new traits.

Item Type: Article
Uncontrolled Keywords: Zea mays L., immature embryos, biolistic genetic transformation, bar, uidA.
Subjects: Genetics
Divisions: Departments > Department of Biochemistry, Medical and Pharmaceutical Chemistry
Depositing User: Оксана Мажуга
Date Deposited: 16 Sep 2020 12:32
Last Modified: 16 Sep 2020 12:32
URI: http://repo.dma.dp.ua/id/eprint/5717

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