Regulation of microrna expression level by choleretictherapy in functional disorders of the gallbladderand оddi’s sphincter in children

Abaturov, Alexandr E. and Vysochyna, Iryna L. and Babych, Veronika L. and Dosenko, Viktor E. (2020) Regulation of microrna expression level by choleretictherapy in functional disorders of the gallbladderand оddi’s sphincter in children. Wiadomości Lekarskie, 1. pp. 41-45. ISSN 0043-5147

Text REGULATION OF MICRORNA EXPRESSION LEVEL BY CHOLERETICTHERAPY IN FUNCTIONAL DISORDERS.pdf Download (223kB)

Abstract

The alm: To study the effect of choleretic therapy on the level of microRNA expression in functional disorders of the gallbladder and Oddi’s sphincter in children. Materials and methods: Fifty patients with functional disorders of the gallbladder and Oddi’s sphincter who received standard therapy in combination with ursodeoxycholic acid, 20 patients – standard therapy without ursodeoxycholic acid, and 20 healthy children were examined. The level of expression of microRNA-378f, microRNA-4311, microRNA-47143p in the blood serum by the method of real-time polymerase chain reaction with reverse transcription according to the protocol TaqMan Gene Expression Assays was performed. Results: It was demonstrated that the activity profile of microRNA-4714-3p was significantly lower in those examined with functional disorders of the gallbladder and Oddi’s sphincter than in practically healthy children (p<0.05). After standard therapy combined with ursodeoxycholic acid in children with functional disorders of the gallbladder and Oddi’s sphincter, the level of expression of microRNA-378f is significantly higher than before therapy (5.23±0.70 SU and 2.02±0.57 SU respectively) (p<0.05). Against the background of standard therapy with the addition of ursodeoxycholic acid or without it, the expression profile of microRNA-4714-3p in the blood serum in children with functional disorders of the gallbladder and Oddi’s sphincter significantly decreased (1.93±0.58 SU and 1,14±0,53 SU respectively) (p<0.05). Conclusions: Ursodeoxycholic acid in children with functional disorders of the gallbladder and Oddi’s sphincter affects the activity of generation of gene regulators of the cellular mechanisms of microRNA-378f and microRNA-4714-3p.

Item Type:	Article
Additional Information:	DOI: 10.36740/WLek202001107
Uncontrolled Keywords:	The functional disorders of the gallbladder and Oddi’s sphincter, microRNA, ursodeoxycholic acid, children.
Subjects:	Pediatrics
Divisions:	Departments > Department of Pediatrics 1 and medical genetics (formerly - Faculty Pediatrics and Medical Genetics) Faculty of Postgraduate Education > Department of Family Medicine FPE
Depositing User:	Оксана Мажуга
Date Deposited:	23 Nov 2020 08:16
Last Modified:	23 Nov 2020 08:16
URI:	http://repo.dma.dp.ua/id/eprint/5973

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