Features of morphological changes in the masseter muscle in diabetes mellitus under conditions of comorbidity Особливості морфологічних змін жувального м’яза при цукровому діабеті в умовах коморбідності

Habren-Syller, A.-N. and Zhurakivska O.Y., O.Y. (2025) Features of morphological changes in the masseter muscle in diabetes mellitus under conditions of comorbidity Особливості морфологічних змін жувального м’яза при цукровому діабеті в умовах коморбідності. Морфологія = Morphologia, 19 (3). pp. 46-54. ISSN 1997-9665

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Abstract

Diabetes mellitus remains one of the major global healthcare challenges due to its rapid growth, the development of complications, and high mortality rates, despite the emergence of novel therapeutic strategies and approaches. One of its complications is diabetic myopathy, which may develop and progress differently under comorbid conditions. The purpose of our study was to determine the patterns of morphological alterations in the masseter muscle of rats in experimental streptozotocin-induced diabetes mellitus (SIDM) under conditions of chronic immobilization stress (CIS). The material for the study consisted of masseter muscles obtained from 20 sexually mature 6-month-old male rats, which were equally divided into 4 groups: group 1 – comorbid pathology (SIDM + CIS), group 2 – SIDM, group 3 – CIS, group 4 –control animals. Tissue samples were collected on day 28 of the experiment. Histological, electron microscopic, and morphometric methods were applied, followed by statistical data analysis. Results and conclusion. It was established that SIDM induces damage to muscle fibers by the mechanism of vacuolar degeneration and colliquative necrosis. These alterations occur against the background of diabetic microangiopathy and result in muscle fibers atrophy. In conditions of hyperglycemia and impaired blood supply in rats of groups 1 and 2, the following changes were observed: a reduction in MFs area by 31–18%; an increase in mitochondrial volume density by 1.8–1.6 times (p<0.05 in all cases). A characteristic feature of muscle fibers damage in the masseter muscle under comorbid conditions was the development of aseptic inflammatory cellular infiltrates, MFs death through necroptosis and necrosis, fragmentation and lysis of myofibrils, leading to a decrease in their volume density by 11 % compared with controls. In rats exposed to CIS, mitochondrial remodeling was observed (elongation, reduced volume, and increased matrix density), accompanied by a 13 % reduction in mitochondrial volume density. Мuscle fibers underwent atrophic damage via ferroptosis, autolysis, and apoptosis, showing a tendency toward atrophy. Thus, CDM and CIS cause atrophy and damage of masseter muscle fibers manifested by vacuolar degeneration, necroptosis, necrosis, and the formation of aseptic inflammatory infiltrates. These alterations develop against the background of impaired muscle perfusion as a result of diabetic microangiopathy.

Item Type:	Article
Additional Information:	DOI: 10.26641/1997-9665.2025.3.46-54 https://morphology.dmu.edu.ua/pro-zurnal/arhiv-nomeriv/2025-vol-19-3
Uncontrolled Keywords:	masticatory muscles, skeletal muscles, stress, diabetes mellitus, hypokinesia, anatomy, ultrastructure, pathol- ogy, histology=жувальний м’яз, скелетний м’яз, стрес, цукровий діабет, гіпокінезія, анатомія, ультраструктура, патологія, гістологія
Subjects:	Pathological anatomy Endocrinology
Divisions:	University periodicals > Morphology
Depositing User:	Аліна Чеботарьова
Date Deposited:	23 Sep 2026 12:28
Last Modified:	23 Sep 2026 12:28
URI:	http://repo.dma.dp.ua/id/eprint/10290

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