Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases

Pismenetskaya, I.U. and Butters, T.D. (2017) Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases. Ukr. Biochem. J., Vol.89 (N 1). pp. 59-70. ISSN 2409-4943


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To verify the idea that extracellular free oligosaccharides might be able to reflect the functional status of the endoplasmic reticulum (ER) and lysosomal-endosomal system, HPLC-profiles of serum-derived free oligosaccharides (FOS) in human healthy aging, acute myeloproliferative neoplasms, and cardiovascular pathologies were compared with intracellular glycans. After plasma deproteinization and FOS purification the oligosaccharides were labelled with anthranilic acid, separated into the neutral and charged with QAE Sephadex (Q25-120) chromatography and analysed using high-performance liquid chromatography (HPLC). The charged FOS were digested with a sialidase and compared with free oligosaccharides from transferrin for structural decoding. HPLC-profiles of serum-derived FOS revealed mild delay of the dolichol phosphate cycle in ER, moderate intensification of ER-associated degradation (ERAD) and degradation in endosomallysosomal system with aging; an inhibition of the dolichol phosphate cycle, intensification of ERAD and increasing of lysosomal exocytosis in acute myeloproliferative neoplasms; intensification of ERAD and glycocojugate degradation with endosomal-lysosomal system in cardiovascular diseases. As serum free oligosaccharides are able to reflect specifically perturbations in ER and endosomal-lysosomal system under wide range of stressors they can serve as extracellular markers of functionality of these organelles.

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Uncontrolled Keywords: free oligosaccharides (FOS), HPLC-profiles of glycans, ER stress, ER-associated protein degradation (ERAD), endosomal-lysosomal disturbances, glycomarkers. вільні олігосахариди, ВЕРХ-спектри гліканів, стрес ЕР, асоційована з ЕР деградація протеїнів, порушення лізосомно- ендосомної системи, глікомаркери. свободные олигосахариды, ВЭЖХ-спектры гликанов, стресс ЭР, ассоциированная с ЭР деградация протеинов, нарушения лизосомно-эндосомной системы, гликомаркеры.
Subjects: Biological Chemistry
Divisions: Departments > Department of Biochemistry and medical chemistry
Depositing User: Елена Шрамко
Date Deposited: 08 Feb 2018 14:43
Last Modified: 08 Feb 2018 14:51

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