Stepanskyi, D.O. and Koshova, I.P. and Kremenchutskyi, G.M. and Khomiak, O.V. (2018) Choosing selective factors for the cultivation of Aerococcus viridans symbiont strains. Regulatory Mechanisms in Biosystems, 9 (3). pp. 426-429. ISSN 2519-8521 (Print), 2520-2588 (Online)
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Abstract
Aerococcus viridans is a bacterium that is present in the environmentand was initially isolated from the air. Other types of Aerococcushave been obtained from different parts of the human body and animals(Collins et al., 1999; Nobukazu Saishu et al., 2009). Aerococci are componentsof normal gut microbiota of animals (Wise & Siragusa, 2007;Senneby et al., 2016) and human intestinal flora. Along with this, thereare cases of allocation of aerosols in humans and animals in variouspathologies and these are associated, as a rule, with immuno-deficiencystates. Initially, the Aerococcus genus, which included a single species –Aerococcus viridans was described. Subsequently, five new Aerococcusspecies: Aerococcus urinae, A. sanguinicola, A. christensenii, A.urinaeequi and A. urinaehominis (Facklam&Elliot, 1995) have beenidentified. The clinical significance of these species was clearlyestablished for A. urinae and A. viridans (Shelton-Dodge et al., 2011;Rasmussen et al., 2013; Humphries et al., 2014; Senneby et al., 2015).A. viridans are ubiquitous microorganisms, and have certain peculiaritiesof cultivation (Williams & Hirch, 1953; Rizhenko et al., 2002;Facklam et al., 2003). Research carried out used displaying mediasthrough which the allocation, identification and differentiation ofaerococci were conducted (Good et al., 1966; Devrise et al., 1999;Kremenchuckij et al., 2003, 2009). The composition of the aforementionedmedia for the selective allocation of various types of aerococciincluded the complex "oxolinic acid – etonium", which gave a selectiveeffect of predominant aercocci growth in concentrations: oxalic acid –50 мg/ml, etonium – 5 мg/ml. In the process of use of these media, dataindicating cases of conditionally pathogenic flora (CPF) contaminationdue to the accumulation of microorganisms that are resistant to the"oxolinic acid – etonium complex" were obtained. The above-mentionedfacts and increased incidence of infections caused by hospital strainswith multi-resistance to chemotherapy (Uh et al., 2002; Yasukawa et al.,2014; Gonzalez et al., 2017), especially methicillin-resistant Staphylococcusaureus (MRSA) (Cho & Chung, 2017), have also created a needto search for new substances that would have a wide range of antagonisticactivity in turn on the CPF and inhibit the growth of aerococci at thesame time and which should also be accessible and safe.The experimental method (Stepanskyi & Kremenchutskyi, 2014) madeit possible to discover the sensitivity of the A. viridans symbionts tofluoroquinolones and determined that norfloxacin at a dosage of8.3 мg/ml practically did not affect the growth of aerococci and effectivelysuppresses the CPF. However, it was important to enhance the selective effect of norfloxacin with other antimicrobial agents in order toreceive stronger suppression of CPF. Therefore, the purpose of the study was to select a selective antibacterial complex that would suppress the growth of UPMF and would not affect the growth of A. viridans, with the possibility of using it for the production of modified media for isolation, cultivation and study of the biochemical activity of A. viridans microorganisms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | aerococci; autosymbionts; cultivation of aerococci; indicator media; modified media. |
| Subjects: | Microbiology |
| Divisions: | Departments > Department of Microbiology, virology, immunology and epidemiology |
| Depositing User: | Ирина Медведева |
| Date Deposited: | 11 Dec 2019 09:24 |
| Last Modified: | 11 Dec 2019 09:24 |
| URI: | http://repo.dma.dp.ua/id/eprint/4674 |
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