Бібікова, В.М. (2014) Особливості геморагічного інсульту у щурів різних вікових груп. Features of hemorrhagic stroke in rats of different age groups. Вісник проблем біології та медицини, Вип. 4 (Т. 3). pp. 87-91.
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Бібікова В.Н.-Вісник проблем біології і медицини.pdf Download (722kB) |
Abstract
Досліджено структурно-морфологічні зміни при первинному та повторному геморрагічному інсульті у щурів зрілого та старечого віку. Трансформація внутрішньомозкової гематоми і перифокальний набряк різко загострюються з віком та кількістю епізодів крововиливу в мозок. При первинному інсульті в корі мозку переважають процеси гідропічної дистрофії нейронів, а при повторному-пікноз, ступінь якого зростає з старечій віковій групі щурів. При повторному геморрагічному інсульті на тлі ангіоневрозу відмічаються вторинні крововиливи в корі мозку та мозолистому тілі, що загострює розвиток набряку мозку та неврологічного дефіциту. Ці дані можуть бути використані з метою вивчення дії лікарських засобів нейропротекторного та протинабрякового механізму дії. Structural and morphological changes at hemorrhagic stroke were investigated in rats of the mature and old age. The transformation of the intracerebral hematoma and perifocal edema sharply escalates with age and with the number of incidents of cerebral hemorrhages. Processes of hydropic dystrophy prevails in the cerebral cortex during the initial stroke, process of pyknosis which stage increases in the old age group of rates during aging prevails at the repeated stroke. When you hemorrhagic stroke on the background angionekrosis observed secondary hemorrhage in the cerebral cortex and the corpus callosum, which aggravates the development of brain edema and neurological deficits. This data can be used to study the effects of neuroprotective and anti-edema drugs. Hemorrhagic stroke is about 20-30 % of all strokes and is a significant medical and social problem. The risk of development of the lethal result at the hemorrhagic stroke increases by 1,5 times, and in elderly, and the old –age people - in 1,8 times. Pathological mechanisms of hemorrhagic stroke continue to discuss in numerous clinical and experimental studies. Worse rates and the less level of functional recovery is stated in most publications, however pathogenic mechanisms and causes of incurable remain discussed. Our article describes the results of morphological disturbances rat brain mature and senile age groups after hemorrhagic stroke. To achieve the goal proposed model of recurrent hemispheric hemorrhagic stroke. The modeling of intracerebral hemorrhage in animals using stereotaxic method and performed mechanical destruction of brain tissue in the region of the internal capsule (capsula interna, L = 3,5-4,0; H = 6,0; AP = 0,6-1,0 ) within the right hemisphere by mandren-knife with additional input in the area of destruction 0,1 ml blood. To simulate recurrent stroke we simulated intracerebral hematoma after 30 days. In 21 days after hemorrhagic stroke we performed histological changes in the rat brain. Histological data of rat brain changes indicate pathologic processes in acute hemorrhagic stroke in elderly rats. Established acute cerebral edema and death of neurons in the cerebral cortex with age. The average number of neurons in the neocortex ipsilateral hemisphere in animals with primary hemorrhagic stroke was 91,6 % in mature rats and 36,8 % in the elderly (p<0,05); at recurrent hemorrhage - 84.5 % and 32.1 %, respectively (p<0,05). During the first stroke in the cerebral cortex prevails cell swelling and necrotic death, after recurrent stroke prevailed cell pyknosis which increases with the senile age These processes are reflected in morphometric parameters of neurons: the initial stroke neurons area increases by 18,7% and 23,1% relatively to mature and old age, and during the recurrent hemorrhage by 23,1% in the first case and decreases by 31,8 % in old rats (p<0,05). Around the hematoma in the internal capsule and corpus callosum was seen sharp brain edema in perivascular and pericellular types. We set stasis and dilatation of blood vessels; in senile rats with recurrent stroke are often recorded secondary hemorrhage in the cerebral cortex and corpus callosum, obviously due to the violation of structural integrity against the backdrop of angionecrosis and age deterioration. Conducted histological analysis showed a significant difference of peripheral edema and focal tissue necrosis of the cerebral hemispheres in primary and recurrent hemorrhagic stroke. Morphological changes increased with age and number of episodes of cerebral hemorrhage. During repeated hemorrhagic stroke with angionecrosis were often registred secondary hemorrhage in the cerebral cortex and corpus callosum that exacerbates the development of brain edema and neurological deficits. The proposed experimental model of recurrent hemispheric hemorrhagic stroke can be used to study the pathogenesis of complications of stroke and effectiveness of drugs with neuroprotective and antiedema mechanism of action.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | геморрагічний інсульт, модель повторного інсульту, вікові зміни.hemorrhagic stroke, recurrent stroke model, age-related changes. |
| Subjects: | Pathological physiology Experimental medicine |
| Divisions: | Departments > Department of Pathological Physiology |
| Depositing User: | Анастасия Жигар |
| Date Deposited: | 11 Apr 2016 10:37 |
| Last Modified: | 11 Apr 2016 10:37 |
| URI: | http://repo.dma.dp.ua/id/eprint/677 |
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