Histochemical characterization of the adrenal glands and testesin cryptorchidism

Sharapova, E.N. (2023) Histochemical characterization of the adrenal glands and testesin cryptorchidism. HEALTH & EDUCATION (4). pp. 82-85. ISSN 2786-7919

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Abstract

In this study, endocrine disorders in the testes and adrenal glands of experimental animals with true cryptorchidism and after orchidopexy were determined. The identification the succinate dehydrogenase, glucose-6-phosphatase, and adenosine triphosphatase in the tissue of dystopic and orthotopic testes of animals was the aim of this research. The methods of enzyme detection by Nakhlas, Walker, Walker-Zeligmman, and Vakhstein-Maizel were used. The research revealed morphological changes in the testes of experimental and control groups, namely: depletion of germ cells, thickening of the basal membrane of seminiferous tubules, compact arrangement of seminiferous tubules in the testes of animals with cryptorchidism – and less pronounced changes in the testicular tissue of control group animals: germ cells at various stages of development clearly covered the walls of seminiferous tubules. SDH activity in the dystopic testis was lower than in the non-descended testis. Also, SDH activity in Sertoli cells and the vascular wall of tubules was lower than in the testes of control animals. That is, in the orthotopic testis, SDH activity is significantly lower than in the dystopic one. The study also showed that in germ cells, vascular walls, and Leydig cells of the dystopic testis, NAD dehydrogenase activity is higher than in the contralateral testis. Glucose-6-phosphatase activity was observed as clusters of brown color in all structures of the testes in both groups. It was proven that in cryptorchidism, the activity of this enzyme was higher in the dystopic testis than in the non-descended one. During the study, it was proven that the adrenal glands also underwent morphological changes, namely: the fascicular zone narrowed and the reticular zone expanded, cells were compactly and disorderly arranged, and the capsule was thickened. In cryptorchidism, SDH activity was higher in the reticular zone than in the medullary zone. NAD-oxidized dehydrogenase increased evenly in all zones of the adrenal glands in the norm, but was less pronounced in cryptorchidism. Glucose-6-phosphatase activity increased in cryptorchidism, while SDH and ATPase activities decreased. The process of moving the testis from the abdominal space to the scrotum was accompanied by a discorrelation of the activity of the zones of the adrenal glands’ cortex, which occurred unevenly in the structures of the adrenal glands. As the study showed, the increased activity of enzymes is the result of the reaction of the sex glands and adrenal glands to the stimulating effect of gonadotropins and corticotropins producing by the anterior pituitary. It can be assumed that in this disease, enzymes in tissues are not eliminated by the body, and their accumulation indicates the extinction of the functional activity of organs, which may lead to the malignant transformation of the testes and adrenal glands in the future.

Item Type:	Article
Additional Information:	DOI: 10.32782/health-2023.4.13
Uncontrolled Keywords:	testis, adrenal gland, NAD dehydrogenase, SDH activity, glucose-6-phosphatase, cryptorchidism
Subjects:	Morphology
Divisions:	Departments > Department of human anatomy, clinical anatomy and operative surgery
Depositing User:	Аліна Чеботарьова
Date Deposited:	06 Jun 2023 10:29
Last Modified:	21 Mar 2024 11:06
URI:	http://repo.dma.dp.ua/id/eprint/8415

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