Дослідження експресії кишкового муцину MUC-2 та маркеру проліферативної активності Кі-67 в колоректальних карциномах

Шпонька, І.С. and Хархаліс, І.К. and Пославська, О.В. (2022) Дослідження експресії кишкового муцину MUC-2 та маркеру проліферативної активності Кі-67 в колоректальних карциномах. Морфологія = Morphologia, 16 (3). pp. 110-117. ISSN 1997-9665

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Abstract

Colorectal carcinomas (CRCs), as defined by WHO, are malignant epithelial tumors originatingin the large intestine, showing glandular or mucinous features of differentiation. The development of CRC demonstrates acomplex pathogenesis due to a violation of the intestinal mucosal barrier. Goblet cells are thought to secrete mucin, whichforms a mucosal barrier and maintains intestinal integrity. Mucin type 2 (MUC-2) is mainly expressed in the small and largeintestine, and disturbances in its production are associated with various inflammatory diseases and carcinomas. Chronic in-flammation leads to cell damage that transforms the inflamed epithelium into low-grade dysplasia, high-grade dysplasia, andfurther into CRC. Also, the literature emphasizes the importance of studying MUC-2 in individual histological types of CRC,which have a different course and prognosis for patient survival. The aim of the study was to evaluate the prognostic signifi-cance of the expression of intestinal mucin MUC-2 depending on the clinical and morphological characteristics and prolifera-tive activity of colorectal carcinomas. Methods. The work examined the clinical and anatomical material of CRC of 37 pa-tients (15 women and 22 men), which was obtained during operations (right hemicolectomy, resection of the sigmoid colon,Hartmann's operation, resection of the transverse colon, resection of the ileum); all of them were treated in the 2nd surgicaldepartment of the Dnipropetrovsk Regional Clinical Hospital in the period from 2019 to 2021. The age of the patients rangedfrom 27 to 82 years; the average age was 61.43±14.90 years. Primary monoclonal antibodies to Ki-67 (sp6, 1:250), MUC2(sp1, RTU) and UltraVision Quanto imaging system, LabVision) were used for immunohistochemical examination. Results.The average intensity of MUC-2 staining showed a significant difference in individual histological types of CRC (the darkeststaining was found in mucinous and ring-shaped cell carcinomas, the lightest in micropapillary adenocarcinoma, p<0.05); andalso, in subgroups with different proliferative potential according to Ki-67 (a decrease in the intensity of MUC-2 expressionwas accompanied by an increase in the proliferation index, p<0.05). The distribution of MUC-2 expression variants – typical(membrane diffuse or membrane focal) or aberrant (cytoplasmic, cytoplasmic-nuclear dot like) showed a significant differ-ence in the age group (with increasing age, the relative number of aberrant MUC-2 expression increased, p<0 .05); and also,in the group with G2, the relative number of aberrant MUC-2 expression was significantly higher compared to G3 (p<0.05).The absolute absence of an aberrant MUC-2 expression variant was noted in the group of CRC with metastases, compared tothe group without metastases, where they were found in 50.00% (10 out of 20); in the group of CRC with a low proliferationindex, compared to the groups of moderate and high proliferation, where they were found in 46.15% (6 out of 13) and28.57% (4 out of 14), respectively; in certain histological types of CRC, namely, mucinous, ring-shaped cell and adenocarci-noma of the adenoma-like type. The largest number of aberrant variants of MUC-2 expression was observed in such histolog-ical types of CRC as medullary carcinoma 66.67% (2 of 3), micropapillary carcinoma 33.33% (1 of 3) and adenocarcinomaNOS 31.58% (6 of 19).

Item Type:	Article
Additional Information:	DOI: 10.26641/1997-9665.2022.3.110-117
Uncontrolled Keywords:	колоректальна карцинома, цифрова морфометрія, MUC-2, Ki-67
Subjects:	Pathological anatomy
Divisions:	Departments > Department of Pathological Physiology
Depositing User:	Аліна Чеботарьова
Date Deposited:	24 Oct 2023 10:55
Last Modified:	24 Oct 2023 10:55
URI:	http://repo.dma.dp.ua/id/eprint/8669

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