Quantitative and qualitative analysis components the myocardium of rats in hypoxia

Kobeza, P.A. (2023) Quantitative and qualitative analysis components the myocardium of rats in hypoxia. Морфологія = Morphologia, 17 (4). pp. 14-21. ISSN 1997-9665

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Abstract

Various types of hypoxia occur during fetal development and early neonatal phases, influenced by factors that complicate pregnancy. Given the frequency of cardiovascular issues, understanding the impact of antenatal hypoxia on newborn hearts and exploring corrective measures is crucial in medical research and practice. Objective. The study focuses on analyzing the quantitative and qualitative composition of contractile cardiomyocytes as the primary cell population in elements of the myocardial contractile apparatus. It involves examining the proliferative variability of components of the myocardial contractile apparatus under conditions of modeled hypoxia of varying types. Additionally, it includes a quantitative and qualitative analysis of the cellular composition of myocardial elements in adult rats and their offspring subjected to various types of hypoxia during prenatal development. Methods. The study was conducted on sexually mature female Wistar rats (4-5.5 months) and their offspring – newborn rats (14, 16, 20 Prenatal and 1 day Postnatal). The Results. Under the influence of chronic hypoxia on the 14th day of prenatal development, different areas of the atrial myocardium demonstrated significantly different thickness values. The values of this indicator in the second experimental group did not significantly differ from the control group values at this term. The effect on the 16th day of prenatal development affected the proliferative activity. Following exposure to chronic hypoxia, this measure in newborn rats significantly increased in RV, and in the LV compared to the previous term. On the 16th day of prenatal development, the thickness values of the atrial and auricular myocardium in animals from the first experimental group did not significantly differ from the values of the previous term and the control group. The myocardial thickness on the 20th day after the influence did not significantly differ compared to the 16th day of prenatal development and compared to the norm. The proliferative activity of atrial cardiomyocytes in animals from the first experimental group gradually decreased until the first day of postnatal development, which was reflected in the reduction in the number of Ki-67 positive cells compared to the 16th day. Conclusion. Under conditions of hypoxia and in normal conditions from the 14th day of prenatal development to the 1st day of postnatal development, the myocardial thickness demonstrates an inverse correlation with the proliferation index of atrial cardiomyocytes; under normal conditions. Hypoxia does not significantly affect the processes of proliferation of atrial cardiomyocytes.

Item Type:	Article
Additional Information:	DOI: 10.26641/1997-9665.2023.4.14-21
Uncontrolled Keywords:	tissue hypoxia, cardiomyocyte, contractile apparatus, ultrastructure, myofibrils
Subjects:	Cardiology Experimental medicine
Divisions:	Departments > Department of histology University periodicals > Morphology
Depositing User:	Аліна Чеботарьова
Date Deposited:	23 May 2024 08:14
Last Modified:	23 May 2024 08:14
URI:	http://repo.dma.dp.ua/id/eprint/8947

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