Changes of rat's brain vesseles after air shock wave exposure

Козлова, Ю.В. and Козлов, С.В. and Маслак, Г.С. and Бондаренко, О.О. and Дунаєв, О.В. and Оберемок, М.Г. (2024) Changes of rat's brain vesseles after air shock wave exposure. Вісник морфології = Reports of Morphology, 30 (1). pp. 55-60. ISSN 1818-1295 (Print), 2616-6194 (Online)

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Abstract

Mild blast-induced traumatic brain injury is common among the military, resulting incognitive impairment, reduced socialization, which leads to disability and, as a result,a deterioration in the quality of life. It is considered that blood-brain barrier disruptionand microvascular dysfunction are the key to this type of injury. The purpose of studywas to study changes in brain vessels after air shock wave exposure. The study wascarried out on 48 mature male Wistar rats, which were randomly divided into 2 groups:an experimental group, in which animals were subjected to inhalation anesthesia usinghalothane and exposed to a shock wave with an overpressure of 26.4±3.6 kPa, and aSham group. After simulation of injury on days 1st, 3rd, 7th, 14th, and 21st, the rats wereeuthanized and the brain was removed and after all subjected to standard histologicalprocedures and stained with hematoxylin and eosin. For immunohistochemical studies,as primary antibodies were used eNOS. The finished preparations were examined bylight microscopy and photographed. Disorders of the cerebral vessels in experimentalrats were detected from day 1st of the posttraumatic period. It was found that the blastwave led to vascular rupture, as well as increased vascular permeability with diapedesisof red blood cells and cerebral edema for up to 21st days. Focal violations of thevascular wall integrity in cortical and hippocampal hemocapillaries, venular link of thesubmembrane vessels; changes in the morphology of the metabolic vesselsendothelium; uneven blood filling of the brain vessels were of major importance. Thesechanges indicate that increased eNOS expression leads to dilation of cerebral vessels,which is a compensatory mechanism in response to injury to improve cerebral bloodcirculation. However, eNOS is not involved in vasodilation, which we observed up to21st day post-trauma.

Item Type:	Article
Additional Information:	DOI: 10.31393/morphology-journal-2024-30(1)-07
Uncontrolled Keywords:	blood-brain barrier, explosion, trauma, brain, blood vessels
Subjects:	Morphology
Divisions:	Departments > Department of Biochemistry and medical chemistry Departments > Department of Pathological Physiology
Depositing User:	Аліна Чеботарьова
Date Deposited:	06 Sep 2024 11:19
Last Modified:	06 Sep 2024 11:19
URI:	http://repo.dma.dp.ua/id/eprint/9067

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