Influence of trace elements changes in cerebellum on rat`s behavior in elevated plus maze in the early period of mild blast-induced traumatic brain injury

Козлова, Ю.В. (2024) Influence of trace elements changes in cerebellum on rat`s behavior in elevated plus maze in the early period of mild blast-induced traumatic brain injury. Патологія = Pathologia, 21 (1). pp. 28-33. ISSN 2306-8027 (print), 2310-1237 (online)

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Abstract

: The aim of the current study was to determine whether there are changes in brain trace elements of rats with blast-inducedtrauma and if these changes affect behavior in the elevated plus maze.Materials and methods. The study was carried out on 126 sexually mature male Wistar and were divided into 3 groups:Experimental – exposed to a shock wave 26.4 ± 3.6 kPa (n = 42); Sham (n = 42), the animals of which were subjected toinhalation anesthesia with halothane and fixation in a horizontal position; and Intact (n = 42). Behavior was study in elevatedplus maze. The duration of presence in the open and closed arms, the number of stands, the duration of grooming wasrecorded in all groups of rats for 3 minutes. After, the animals were euthanized with halothane, followed by removal of thebrain. The cerebellum was completely separated for spectral analysis using energy dispersive X-ray fluorescence analysis(EDRFA) on the analyzer EXPERT 3 XL.Results. Results showed significant changes of cognitive activity in experimental group which are indicate functional disor-ders of the cerebellum in the form of maladaptation in space with subsequent inhibition of motor centers. Cu/Fe ratio wasdecreased in the 14 th and 21 st days and increased in the 28 th . Cu/Zn ratio was decreased on the 14th day. Zn/Fe ratio washigher on the 14 th and 28 th days. The existence of correlations between changes in trace elements and behavioral disordersin experimental rats was established.Conclusions. In the early period of blast-induced traumatic brain injury, cerebellar dysfunction in the form of spatial maladap-tation with subsequent depression of motor centers was observed in the experimental rats Correlation analysis showed thepresence of different strengths and directions of relationships between the ratios of Cu/Fe, Cu/Zn and Zn/Fe in the cerebellumand behavioral indicators in the elevated plus maze (duration of stay in open and closed arms, grooming and vertical motoractivity) of experimental rats.elements and behavioral disordersin experimental rats was established.Conclusions. In the early period of blast-induced traumatic brain injury, cerebellar dysfunction in the form of spatial maladap-tation with subsequent depression of motor centers was observed in the experimental rats Correlation analysis showed thepresence of different strengths and directions of relationships between the ratios of Cu/Fe, Cu/Zn and Zn/Fe in the cerebellumand behavioral indicators in the elevated plus maze (duration of stay in open and closed arms, grooming and vertical motoractivity) of experimental rats.

Item Type:	Article
Additional Information:	DOI: 10.14739/2310-1237.2024.1.296887
Uncontrolled Keywords:	traumatic braininjury, traceelements, elevatedplus maze,cerebellum
Subjects:	Pathological physiology
Divisions:	Departments > Department of Pathological Physiology
Depositing User:	Аліна Чеботарьова
Date Deposited:	14 Oct 2024 08:49
Last Modified:	14 Oct 2024 08:49
URI:	http://repo.dma.dp.ua/id/eprint/9129

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