Сакович, В.Н. (2015) Значение лабораторной диагностики герпетического кератита. Дерматовенерология. Косметология. Сексопатология, № 1-2. pp. 41-45.
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Abstract
В работе приведены данные по изучению информативности ДНК-анализа слезы методом ПЦР при разных типах герпетического кератита. Пациенты с герпетическим кератитом были разделены на 3 группы: 1. Эпителиальный кератит (древовидный и географический) – 40 человек; 2. Стромальный кератит – 36 человек; 3. Эндотелиит – 10 человек. Помимо общепринятых офтальмологических обследований, всем пациентам до начала лечения проводился анализ слезы методом ПЦР на наличие ДНК ВПГ 1 и 2 типа. Во всех 40 случаях эпителиального кератита в слезе определялась ДНК ВПГ тип 1 (100%). В 16 случаях стромального кератита так же определялся ВПГ тип 1 (44,4%). ВПГ не определялся в случаях эндотелиита. Ни в одном из случаев не определялся ВПГ тип 2. Наблюдалась хорошая корреляция типичной клинической картины герпетического кератита с выявлением вирусной ДНК, что говорит о репродукции вируса. Introduction. The most part of human population is infected by virus herpes simplex. This infection is often symptom-free, but ophthalmological form of disease leads to complicated pathology with serious corneal damage. Virus herpes simplex is in the lead cause of blindness in developed countries. There are several types of laboratory diagnostics: 1.Polymerase chain reaction; 2. Serological investigations; 3. Cultural method; 4. Bioassay. Polymerase chain reaction is the gold standart of clinical diagnostics, is very reliable in the cases of superficial forms of keratitis, but only in half cases helps to detect virus in material. Serological investigations may be useful to. Cultural method and bioassay are the rare types of diagnostics for modeling disease in laboratory animals. Objective. The goal was to study peculiarities of laboratory diagnostics of herpetic keratitis by polymerase chain reaction in different types of herpetic keraritis. Materials and methods. Due to last classification of Herpetic keratitis Infection Research Group (Davison et al., 2005) 86 patients were divided into 3 groups: 1. Epithelial keratitis−40 patients; 2. Stromal keratitis−36 patients; 3. Endothelitis−10 patients. Before treatment tears of all patients were investigated by polymerase chain reaction to detect herpes simplex virus type 1 & 2 (HVS). Results and discussion. All 40 patients with epithelial keratitis were herpes simplex virus type 1 positive. 16 patients with stromal keratitis were also herpes simplex virus type 1 positive. Virus herpes was not detected in cases of endothelitis. Herpes simplex virus type 2 did not determine at all. There was a good correlation of typical clinical picture and detection of herpes simplex virus. Conclusions. 1. PCR-diagnostics is commercial accessible, fast and reliable method in the cases of epithelial keratitis and some cases of stromal keratitis. It is important not only for treatment strategy but also for accurate monitoring. 2. In cases of stromal keratitis when epithelium is not damaged it is ideally to add serological methods, because probability of HVS detection is smaller. We concider that investigation of Ig A, M, G may be useful.
| Item Type: | Article |
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| Uncontrolled Keywords: | герпетический кератит, вирус простого герпеса 1 и 2 тип, лабораторная диагностика, полимеразная цепная реакция. herpetic keratitis, herpes simplex virus type 1 & 2, laboratory diagnostics, polymerase chain reaction. |
| Subjects: | Ophthalmology |
| Divisions: | Departments > Department of Neurology |
| Depositing User: | Анастасия Жигар |
| Date Deposited: | 18 Nov 2016 07:46 |
| Last Modified: | 18 Nov 2016 07:46 |
| URI: | http://repo.dma.dp.ua/id/eprint/1199 |
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