Immunohistochemical Features of the Basal Layer of the Endometrium: Morphological Aspects

Khaskhachykh, D.A. and Potapov, V.O. and Poslavska, O.V. (2023) Immunohistochemical Features of the Basal Layer of the Endometrium: Morphological Aspects. Journal of Gynecol Women’s Health, 25 (4). 0001-0010. ISSN ISSN 2474-7602

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Abstract

Background: The article examines the morphological and immunohistochemical characteristics of all layers of the endometrium in women of reproductive age with endometrial hyperplasia without atypia in comparison with secretory endometrium, who had abnormal uterine bleeding (AMB). Purpose: To study the morphological and immunohistochemical features of the structure of all layers of the endometrium, divided into functional and nonfunctional zones, which may have an impact on the development of endometrial hyperplasia. Patients and Methods: The study was performed on endometrial morphological material obtained by diagnostic biopsy from 21 women with abnormal uterine bleeding (ABB) in the gynecological department of the Dnipro Clinical Hospital No. 9 in Dnipro during 2022-2023. The criteria for inclusion in the studied cohort of patients were: the age of women from 32 to 45 years (38.4±2.55), the presence of endometrial hyperplasia without atypia, or secretory changes of the endometrium according to the results of histological examination, the exclusion criteria were: the presence of inflammatory diseases of the pelvic organs , tumor pathology of the uterus and ovaries, endometriosis of the uterus, severe somatic pathology, any form of endocrinopathy and metabolic syndrome. The average body mass index was 27.83±1.96 kg/cm2. The study included morphological and immunohistochemical (IHC) research methods. Endometrial tissue was obtained by endometrial curettage, which was performed in women with abnormal uterine bleeding. Primary antibodies to ER (sp1, RTU), PgR (YR85, 1:200), CK 7 (sp1, RTU), CK8 (sp1, RTU), p53 (E247, RTU) and the UltraVision Quanto imaging system (LabVision). Statistical processing of the obtained results was carried out using the Office 365 A1 for faculty software No. 1003BFFD8C8E8B0D. Arithmetic mean (M) and standard deviation (SD) values were calculated. The non-parametric test χ² was used to compare the distribution of features in groups. The difference was considered statistically significant at p0, 05). 2. The proliferative activity of endometrial cells according to the Ki-67 marker increased from superficial to basal parts and showed a statistically significant difference between endometrial hyperplasia samples without atypia and secretory endometrium (p0.05).

Item Type:	Article
Additional Information:	DOI: 10.19080/JGWH.2023.25.556168
Uncontrolled Keywords:	Endometrium; Receptors; Endometrial hyperplasia; Immunohistochemistry; Estrogen receptors; Progesterone receptors; ER; PgR; Ki67; CK7; CK8
Subjects:	Pathological anatomy
Divisions:	Departments > Department of Pathological Physiology
Depositing User:	Аліна Чеботарьова
Date Deposited:	23 Oct 2023 12:03
Last Modified:	24 Oct 2023 06:51
URI:	http://repo.dma.dp.ua/id/eprint/8667

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