Quantitative analysis of vascularization, proliferation and expression of androgen receptors by epithelial skin cells in subversive abscessing perifolliculitis of the scalp

Poslavska, O.V. and Statkevych, О.І. and Svyatenko, T.V. (2025) Quantitative analysis of vascularization, proliferation and expression of androgen receptors by epithelial skin cells in subversive abscessing perifolliculitis of the scalp. Морфологія, 19 (4). pp. 74-80. ISSN 1997-9665

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Abstract

Hoffmann's disease or abscessing perifolliculitis of the scalp is a chronic, therapy-resistant and recurrent purulent-inflammatory disease of the hair follicles, which causes the formation of deep abscesses, cicatricial alopecia and significant changes in the scalp. The disease occurs mainly in young men aged 20–40 years. According to the statistics of the medical center of the private enterprise "Dzerkalo", Dnipro, Ukraine, over the past ten years in Ukraine, the number of cases of abscessing perifolliculitis has begun to increase among men, especially military personnel, due to the wearing of specific protective equipment and additional factors of occlusion and inflammation of hair follicles. The aim of the work is to investigate stromal vascularization, proliferation and expression of androgen receptors in the epithelium of the scalp using the Fiji platform in subversive abscessing perifolliculitis of the head. Methods. Biopsy material from patients diagnosed with abscessing perifolliculitis of the head (Hoffmann's disease) who underwent examination and treatment at the medical center of the private enterprise "Dzerkalo", Dnipro, Ukraine was studied. All patients were men, their age ranged from 20 to 51 years, the average age was 35.5±11.54 years. IHC was performed according to the TermoScientific (TS) protocols with primary antibodies to vascular endothelial cells CD34 (sp1, RTU), Ki-67 (sp6, 1:250), AR (sp1, RTU). The Lab Vision Quanto imaging system (TS, USA) was used with the reaction determination using DAB Quanto Chromogen (TS, USA). Results. The average number of CD-34-positive vessels showed a significant difference in the samples of subducting abscessing perifolliculitis of the scalp, compared with the control group in all studied localizations (subepidermal location, deep layers of the dermis, areas around hair follicles, areas around sebaceous glands (all p<0.05), with the highest number of 24.33±3.78 in areas around hair follicles. The average perimeter of CD-34-positive vessels showed a significant difference in the samples of subducting abscessing perifolliculitis of the scalp, compared with the control group in the deep layers of the dermis, areas around hair follicles and sebaceous glands (p<0.05), with the highest value of 85.83 ±10.52 in areas around hair follicles. The Ki-67 proliferation index in reactive stratified squamous epithelium at in subversive abscessing perifolliculitis of the head was 23.46±4.24%, which was statistically significantly different from the control group (p<0.05), whose proliferation rate remained at the level of 5.22±1.58%. In contrast, the expression of androgen receptors in the study and control groups did not show any difference (94.32±15.08% and 82.40±10.26%, respectively, p>0.05), which refutes the theory of hormonal dependence of chronic inflammation in this pathology.

Item Type:	Article
Additional Information:	DOI: 10.26641/1997-9665.2025.4.74-80
Uncontrolled Keywords:	vascularization, Cd34, Ki-67, androgen receptors, abscessing perifolliculitis of the head, Hoffmann's disease, cicatricial alopecia, dermatology
Subjects:	Dermatology. Skin diseases
Divisions:	Departments > Department of Pathological Physiology Departments > Department of Skin and Venereal Diseases University periodicals > Morphology
Depositing User:	Аліна Чеботарьова
Date Deposited:	31 Mar 2026 09:07
Last Modified:	31 Mar 2026 09:07
URI:	http://repo.dma.dp.ua/id/eprint/9763

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