Microbiological features of Staphylococcus aureus isolated from respiratory tract of children with cystic fibrosis

Ishchenko, Oksana and Koshova, Iryna and Borysova, Inna and Stepanskyi, Dmytro (2021) Microbiological features of Staphylococcus aureus isolated from respiratory tract of children with cystic fibrosis. Wiadomości Lekarskie, 74 (9 p.I). pp. 2094-2099. ISSN 0043-5147 (Print), 2719-342X (Online)

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Abstract

The aim: To determine the prevalence rate of Staphylococcus aureus infection among children with Cystic Fibrosis in the Dnieper region, to provide microbiological characteristics of the isolates and to elevate their susceptibility to antimicrobials. Materials and methods: Sputum, tracheobronchial lavage waters and/ or deep smear from the posterior pharyngeal wall were taken from children with genetically confirmed Cystic Fibrosis. Bacteriological method was the main. The first screening for small colony variants of Staphylococcus aureus was carried out after 48 hours of incubation. The antimicrobials susceptibility testing was determined by disk-diffusion method according to the EUCAST 2019. Microsoft Office Excel 2010 was used for statistical data processing. Results: Twenty one children were enrolled in the survey. The culture of Staphylococcus spp. was obtained from all patients with 40.8% positive for Staphylococcus aureus. Small colony variants appeared with the prevalence rate 21.6% after 48 hours of incubation. The frequency of associations between Staphylococcusaureus with auxotroph phenotype with the presence of Pseudomonasaeruginosa was significantly higher than with wild-type group. The 3d-generation aminoglycosides, the 3d-generation fluoroquinolones, linezolid, rifampicin and tetracyclines showed the best antimicrobial activity, however, resistance to cefoxitin and gentamicin was significantly higher in auxotroph-modified group. Conclusions: Infection Staphylococcusaureus is common among children. The appearance of auxotrophs registered after treatment with aminoglycosides and/ or co-trimoxazole and co-infection Pseudomonas aeruginosa. Isolates of Staphylococcus aureus showed good chemotherapeutic sensitivity, but tendency in increasing resistance registered for auxotroph-modified phenotype.

Item Type:	Article
Additional Information:	DOI: 10.36740/WLek202109112 https://www.scopus.com/record/display.uri?eid=2-s2.0-85121938851&origin=resultslist&zone=contextBox
Uncontrolled Keywords:	cystic fibrosis, Staphylococcus aureus, small colony variants, chronic respiratory tract infection
Subjects:	Microbiology, Virology and Immunology
Divisions:	Departments > Department of Microbiology, virology, immunology and epidemiology Faculty of Postgraduate Education > Department of Medical and Social Expertise and Rehabilitation FPE
Depositing User:	Елена Шрамко
Date Deposited:	02 Jan 2023 10:53
Last Modified:	02 Jan 2023 10:53
URI:	http://repo.dma.dp.ua/id/eprint/8042

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